Anti-RTKIN / Rhotekin Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BST9

Core Product Specifications and Parameters

Biological Background: Rhotekin Function and Localization

• Rhotekin (RTKN) is a protein encoded by the RTKN gene in humans, also referred to as RTKN1. Alternative splicing generates multiple transcript variants.
• It acts as a downstream mediator of Rho GTPase signaling, activating the NF-kappa-B transcription factor, which may promote apoptosis resistance, particularly in gastric tumorigenesis.
• RTKN may also play a role in organizing septin cytoskeletal structures.
• The protein is highly expressed in prostate tissue, with moderate levels in kidney, heart, brain, spleen, testis, placenta, small intestine, pancreas, skeletal muscle, and peripheral blood leukocytes; low expression is observed in ovary, colon, and thymus.
• RTKN is weakly expressed in normal cell lines but overexpressed in various cancer cell lines, suggesting a role in oncogenesis.
• Post-translational modifications include phosphorylation and methylation, and the protein features coiled-coil and GTP-binding domains.

Experimental Guidance and Technical Tips

• For Western blotting, detect a band at approximately 63 kDa (predicted molecular weight 62.7 kDa) in relevant tissue or cell lysates; use a reduced loading buffer and appropriate controls.
• In immunofluorescence/immunocytochemistry, optimize fixation and permeabilization conditions; RTKN may localize to the cytoplasm or associate with septin filaments.
• The antibody cross-reacts with mouse and rat, so validate in the desired model system; sequence homology in the immunogen region supports this cross-species recognition.
• For ELISA, consider sandwich or indirect formats using the recombinant immunogen as a positive reference standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated one-stop sourcing partner for IVD raw materials, from monoclonal and polyclonal antibodies to bulk ancillary reagents and rare target sourcing. This anti-RTKIN polyclonal antibody supports research into Rho signaling, NF-kappa-B activation, and gastric cancer, and we can supply matched antibody pairs or optimized antibodies for your assay development. Reach out to learn how we can streamline your diagnostic raw material procurement—from concept to clinic.