Anti-RTCB Rabbit Polyclonal Antibody for WB, ELISA - Q9Y3I0

Core Product Specifications and Parameters

Biological Background: RTCB Function and Localization

• RTCB (RNA-splicing ligase RTCB), also known as 3'-phosphate/5'-hydroxy nucleic acid ligase or RNA 2',3'-cyclic phosphate and 5'-OH ligase, is the catalytic subunit of the tRNA-splicing ligase complex (tRNA-LC).
• It functions as a 3'-5' RNA ligase essential for the maturation of intron-containing pre-tRNAs, joining exon halves after intron removal by tRNA-splicing endonuclease via a GTP- and Mn²⁺-dependent three-step mechanism (PubMed:40069351, PubMed:24870230, PubMed:38493148). Related references: PMID:40069351 PMID:24870230 PMID:38493148
• RTCB also mediates non-canonical cytoplasmic splicing of XBP1 mRNA during the unfolded protein response (UPR); upon ER stress, IRE1 excises a short intron, and RTCB ligates the exons to produce the active transcription factor XBP1s (PubMed:25378478, PubMed:26483401, PubMed:38493148). Related references: PMID:25378478 PMID:26483401 PMID:38493148
• The protein localizes to both the nucleus and cytoplasm, consistent with its roles in tRNA processing and cytoplasmic XBP1 splicing.
• Key structural and regulatory features include a GTP-binding site, metal-binding residues for Mn²⁺, and modification by phosphorylation and ubiquitin-like conjugation, as indicated by UniProt keywords.
• RTCB belongs to the RtcB family of RNA ligases and is conserved across human, mouse, and rat, supporting cross-species antibody reactivity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 236–505), which is highly conserved across human, mouse, and rat, consistent with the antibody’s predicted cross-reactivity.
• For Western blotting, a specific band around 55 kDa is expected in lysates from human, mouse, and rat cells or tissues; endogenous expression levels may vary.
• In ELISA applications, consider using the antibody as a capture or detection reagent after verifying binding in the intended assay format.
• Validate antibody performance in relevant biological systems, such as cell lines undergoing ER stress to induce XBP1 splicing or models requiring tRNA maturation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated sourcing partner for IVD raw materials, supporting diagnostic developers from concept to clinic. This RTCB antibody is positioned for research in RNA splicing and unfolded protein response pathways, and we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare or custom targets, our team assists with sourcing tailored raw materials to accelerate your assay development.