Anti-RTBDN/Retbindin Rabbit Polyclonal Antibody for WB, ELISA - Q9BSG5

Core Product Specifications and Parameters

Biological Background: Retbindin Function and Localization

• Retbindin (RTBDN) is a riboflavin-binding protein that may serve as a flavin carrier in the retina, potentially facilitating transport across the interphotoreceptor matrix.
• Encoded by the RTBDN gene, the protein exists in a single secreted isoform following signal peptide cleavage; alternative splicing has been noted.
• Localizes to the extracellular matrix and interphotoreceptor matrix of the retina, and is also found at the cell membrane; consistent with secreted and membrane-associated forms.
• Predominantly expressed in the peripheral retina at the protein level, suggesting a specialized role in outer retinal regions.
• Contains a disulfide bond and a signal peptide, supporting its secretion and structural stability in the extracellular environment.
• Keywords: alternative splicing, cell membrane, disulfide bond, extracellular matrix, membrane, secreted, signal, reference proteome.

Experimental Guidance and Technical Tips

• The immunogen, comprising amino acids 63–261, covers the mature protein lacking the signal peptide; thus, the antibody may recognize both precursor and mature forms. Consider validating in retinal tissue lysates or interphotoreceptor matrix preparations.
• Predicted molecular weight is approximately 24.6 kDa (UniProt), though migration at ~25 kDa may be observed by WB due to minor modifications; use appropriate positive controls (e.g., retinal extracts).
• Suitable for Western blot (WB) and ELISA applications; cross-reactivity with rat has been reported, enabling studies in rodent models. Optimize antibody dilution for each assay.
• For ELISA, consider coating with recombinant retbindin or using capture/detection antibody pairs; note that the polyclonal nature allows detection of multiple epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, technical services, and consulting to support every stage of diagnostic development. This anti-retbindin polyclonal antibody is part of our portfolio for retinal biology research and flavin transport studies. For large-scale or customized needs, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—helping diagnostic manufacturers and labs transition seamlessly from concept to clinic.