Anti-RSRC1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96IZ7

Core Product Specifications and Parameters

Biological Background: RSRC1 Function and Localization

• RSRC1, also known as Serine/Arginine-related protein 53 (SRrp53) or Arginine/serine-rich coiled-coil protein 1, is a member of the serine/arginine-rich protein family involved in mRNA processing.
• It has a role in alternative splicing and transcription regulation, as shown by functional studies. Related references: PMID:29522154
• RSRC1 is involved in both constitutive and alternative pre-mRNA splicing, and may participate in recognition of the 3' splice site during the second step of splicing.
• Subcellularly, the protein localizes primarily to the nucleus and nuclear speckles, with additional presence in the cytoplasm.
• It is widely expressed across tissues, with notable levels in the brain, spinal cord, and cerebellum.
• Post-translational modification includes phosphorylation, consistent with its annotation as a phosphoprotein.
• Genetic variants in RSRC1 are associated with intellectual disability, underscoring its neurodevelopmental importance.

Experimental Guidance and Technical Tips

• For western blotting, consider using reducing and denaturing conditions; the expected molecular weight is approximately 39 kDa. Validate band specificity using positive and negative control lysates from human, mouse, or rat samples.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), appropriate antigen retrieval and permeabilization steps may be necessary. Optimize antibody dilution and detection conditions using known positive tissue sections (e.g., brain or spinal cord).
• For ELISA, a checkerboard titration is recommended to determine the optimal coating antigen and antibody concentration. Use the immunogen sequence as a reference for antigen preparation.
• As a polyclonal antibody, batch-to-batch consistency should be confirmed if multiple lots are used in longitudinal studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a streamlined, one-stop channel for IVD raw-material procurement. Beyond this RSRC1 antibody—suitable for splicing and transcription research—we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. Our portfolio supports every stage from concept to clinic, ensuring reliable supply for your assay development needs.