Anti-RSPO1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q2MKA7

Core Product Specifications and Parameters

Biological Background: RSPO1 Function and Localization

• RSPO1 (R-spondin-1) is a secreted activator of the canonical Wnt signaling pathway, functioning as a ligand for LGR4, LGR5, and LGR6 receptors to potentiate Wnt signal transduction (PubMed:29769720). Related references: PMID:29769720
• It also inhibits ZNRF3, a negative regulator of Wnt signaling, and acts as a direct ligand for frizzled FZD8 and LRP6.
• RSPO1 regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through interaction with KREM1 (PubMed:17804805). Related references: PMID:17804805
• The protein may negatively regulate the TGF-β pathway and plays an essential role in ovary determination.
• Subcellular localization includes secretion into the extracellular space and presence in the nucleus.
• Tissue expression is abundant in adrenal glands, ovary, testis, thyroid, and trachea, with low or absent expression in bone marrow, spinal cord, stomach, leukocytes, colon, small intestine, prostate, thymus, and spleen.
• Key structural features include disulfide bonds, glycosylation, heparin-binding domains, and a furin-like repeat domain; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the central region (aa 90-210) of human RSPO1, a region conserved across species, which supports the observed cross-reactivity with mouse and rat.
• For Western blot, the predicted molecular weight is ~29 kDa (unprocessed) or ~26 kDa (mature form), but glycosylation may shift the apparent band; validate using the appropriate positive control lysates.
• In IHC-P and IF/ICC, consider permeabilization steps to detect nuclear-localized RSPO1; fixation and antigen retrieval methods should be optimized for your sample type.
• For ELISA applications, pairing with a capture antibody or using this antibody for detection may be suitable; spike-and-recovery experiments are recommended to assess matrix effects in serum/plasma samples.
• All experimental conditions, including dilutions and incubation parameters, should be empirically determined in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw material procurement. For Wnt signaling research targeting RSPO1, we supply reliable antibodies like this anti-RSPO1 pAb, along with technical consultation and custom sourcing services to accelerate your diagnostic development from concept to clinic.