Anti-RSAD2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8WXG1

Core Product Specifications and Parameters

Biological Background: RSAD2 Function and Localization

• RSAD2 (viperin, cig5, vig1) is an interferon-inducible antiviral protein that establishes a broad cellular antiviral state in response to type I and type II interferons. Related references: PMID:31812350
• It functions as an S-adenosylmethionine-dependent nucleotide dehydratase, converting CTP to ddhCTP. This metabolite acts as a chain terminator for viral RNA-dependent RNA polymerases, directly inhibiting replication of numerous viruses including HCMV, HCV, WNV, dengue virus, influenza A, and HIV-1. Related references: PMID:29925952 PMID:30872404 PMID:30587778 PMID:30934824
• RSAD2 potentiates innate immune signaling by facilitating Lys-63-linked ubiquitination of IRAK1 via TRAF6, thereby enhancing TLR7/9-dependent IFN-beta production in plasmacytoid dendritic cells. Related references: PMID:30872404
• In adaptive immunity, RSAD2 modulates CD4+ T-cell activation and Th2 differentiation by regulating NFKB1 and JUNB activities, thereby promoting GATA3 activation and Th2 cytokine production.
• The protein exhibits a multifaceted subcellular distribution, localizing to the endoplasmic reticulum membrane, Golgi apparatus, lipid droplets, and mitochondria (both inner and outer membranes), allowing it to interface with diverse cellular pathways.
• Structural hallmarks include a radical SAM domain with a 4Fe-4S cluster, and the protein undergoes post-translational modifications such as acetylation and ubiquitination, consistent with its roles in antiviral defense, innate immunity, and lipid metabolism.

Experimental Guidance and Technical Tips

• The immunogen spans residues 118–361, covering the core radical SAM domain, which is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, endogenous RSAD2 runs at ~42 kDa; however, its expression is low under basal conditions. Induction with type I interferon (e.g., IFN-α) or viral stimuli is recommended to robustly detect the protein.
• In immunofluorescence, RSAD2 can localize to the ER, Golgi, and lipid droplets; co-staining with organelle-specific markers (e.g., calnexin, GM130, BODIPY) aids in accurate interpretation.
• For ELISA, use recombinant RSAD2 protein or lysates from interferon-treated cells as a positive control. Dilutions and coating conditions must be optimized empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For RSAD2-related research and immunoassay development, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and bespoke sourcing for rare targets. Whether you need high-quality anti-RSAD2 antibodies for antiviral screening or custom immunoassay development, CamelBio bridges your discovery to diagnostic deployment.