Anti-RRP8 Polyclonal Antibody for WB, ELISA - O43159

Core Product Specifications and Parameters

Biological Background: RRP8 Function and Localization

• RRP8 (Ribosomal RNA-processing protein 8), also known as Nucleomethylin or Cerebral protein 1, is a nucleolar protein that functions as a key component of the energy-dependent nucleolar silencing (eNoSC) complex.
• The eNoSC complex mediates transcriptional silencing of ribosomal DNA (rDNA) in response to cellular energy status, linking metabolism to rRNA expression.
• Upon glucose starvation, elevated NAD+/NADP+ activates SIRT1, which deacetylates histone H3, enabling SUV39H1-mediated dimethylation of H3K9 (H3K9me2); RRP8 binds to H3K9me2, facilitating silent chromatin formation at the rDNA locus.
• RRP8 is predicted to possess methyltransferase activity, though its substrates remain unidentified, suggesting potential roles in chromatin modification.
• Subcellularly, RRP8 localizes predominantly to the nucleus, specifically the nucleolus, consistent with its role in rDNA regulation.
• The protein is subject to phosphorylation and is classified as a chromatin regulator, methyltransferase, transcriptional repressor, and rRNA processing factor.

Experimental Guidance and Technical Tips

• For Western blotting, use standard protocols with appropriate loading controls; the predicted molecular weight is 51 kDa, but actual migration may vary.
• Validate antibody specificity in your experimental system by using positive controls (e.g., human, mouse, or rat cell lines expressing RRP8) and consider knockdown or knockout validation.
• Due to the use of a peptide immunogen, confirmation of specificity via blocking peptide competition is recommended.
• For ELISA, optimize coating conditions and antibody dilutions empirically; the antibody may be suitable for indirect or sandwich ELISA setups depending on available capture/detection pairs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials and technical services, covering every stage from concept to clinic. For studies on rDNA silencing and epigenetic regulation, we offer validated antibodies, optimized monoclonal/polyclonal antibody pairs, and bulk ancillary reagents. Our sourcing capabilities extend to rare target raw materials, ensuring a reliable supply for your RRP8-related research and assay development.