Anti-RRP36 Polyclonal Antibody for WB and ELISA - Q96EU6

Core Product Specifications and Parameters

Biological Background: RRP36 Function and Localization

• RRP36 (Ribosomal RNA processing protein 36 homolog) is a nucleolar protein essential for ribosome biogenesis.
• It functions specifically in the early cleavage steps of pre-rRNA that ultimately yield the mature 18S rRNA of the small ribosomal subunit.
• The protein localizes predominantly to the nucleolus, the subnuclear compartment dedicated to rRNA transcription and ribosome assembly.
• A predicted coiled-coil domain in RRP36 likely mediates interactions with other nucleolar factors within the pre-ribosomal processing complex.
• Proteomic studies have identified RRP36 as a phosphoprotein, suggesting that post-translational modification may modulate its activity during cell cycle or stress responses.
• Alternative splicing of the RRP36 gene generates multiple transcript variants, which could contribute to functional diversity or regulatory control.

Experimental Guidance and Technical Tips

• The immunogen encompasses nearly the full-length human RRP36 protein (amino acids 1-259), which may elicit antibodies targeting multiple epitopes.
• As a polyclonal antibody, it can be used for Western blot detection and ELISA-based assays; consider confirming specificity on endogenous protein from human cell lysates.
• For Western blot, validate the antibody at a range of dilutions to optimize signal-to-noise ratio in your specific experimental system.
• The predicted molecular weight is ~30 kDa, but verify actual migration patterns using appropriate molecular weight markers and relevant human tissue or cell line controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, CROs, and research institutions with streamlined access to high-quality IVD raw materials for ribosome biology, nucleolar research, and rRNA processing targets. Our supply platform offers validated polyclonal antibodies, recombinant proteins, and ancillary reagents to support immunoassay development and biomarker studies. For rare or custom-specificity targets like RRP36, we leverage a global supplier network to secure the raw materials you need—from discovery to clinical validation.