Anti-RRP1 Rabbit Polyclonal Antibody for WB and ELISA - P56182

Core Product Specifications and Parameters

Biological Background: RRP1 Function and Localization

• RRP1 (Ribosomal RNA processing protein 1 homolog A, also known as NNP-1, Nop52) is a nucleolar protein essential for the generation of 28S rRNA, a critical step in ribosome biogenesis.
• The protein is predominantly localized to the nucleolus within the nucleus, consistent with its role in rRNA processing.
• RRP1 is ubiquitously expressed across fetal and adult human tissues, underscoring its fundamental importance in cellular growth and proliferation.
• RRP1 undergoes post-translational modifications, including phosphorylation and methylation, as indicated by proteomics data.
• It is listed as a reference proteome component and has been identified in numerous proteomics studies.
• The protein has a calculated molecular weight of ~53 kDa (52839 Da), consistent with the observed band in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 26–253) is highly conserved across human, mouse, and rat, supporting the reported cross-reactivity; however, we recommend validating the antibody in your specific species and sample type.
• For Western blot analysis, a predominant band at ~53 kDa is expected. Use appropriate loading controls and consider that post-translational modifications might cause slight shifts in apparent molecular weight.
• This polyclonal antibody can be used for ELISA; optimize coating concentration and detection conditions empirically to achieve the best signal-to-noise ratio.
• As with all research-grade antibodies, verify lot-to-lot consistency and perform appropriate controls for each new experimental batch.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering a comprehensive portfolio of validated antibodies and ancillary reagents. In the field of ribosomal biology and nucleolar research, we provide high-quality tools like this RRP1 polyclonal antibody, along with matched antibody pairs and bulk reagents tailored to your assay development needs. Our expertise ensures seamless access to rare targets and custom sourcing for diagnostic manufacturers and research laboratories.