Anti-RRM2B Rabbit Polyclonal Antibody for ELISA and IF/ICC - Q7LG56

Core Product Specifications and Parameters

Biological Background: RRM2B Function and Localization

• RRM2B (p53R2) is a TP53-inducible ribonucleotide reductase subunit that plays a pivotal role in DNA damage repair by supplying deoxyribonucleotides in a p53-dependent manner.
• It forms an active ribonucleotide reductase (RNR) complex with RRM1, which is expressed in both resting and proliferating cells upon DNA damage.
• The enzyme contains an iron-tyrosyl free radical center essential for catalytic activity, classifying it as an iron-binding oxidoreductase.
• Subcellular localization is detected in both the cytoplasm and nucleus, consistent with its role in DNA repair processes.
• Tissue expression is widespread, with high levels in skeletal muscle, weak expression in thymus, and positive staining reported in epithelial dysplasias and squamous cell carcinomas.
• Genetic variants in RRM2B are associated with mitochondrial DNA depletion syndromes, deafness, neuropathy, and progressive external ophthalmoplegia.

Experimental Guidance and Technical Tips

• The immunogen comprises a synthetic peptide derived from the N-terminal region (amino acids 1–100) of human RRM2B; consider this epitope location when designing experiments.
• This antibody is recommended for ELISA and immunofluorescence/immunocytochemistry (IF/ICC) applications as indicated in the specifications.
• Optimal working dilutions must be determined empirically in the end-user’s own experimental system.
• For IF/ICC, optimize fixation and permeabilization protocols to detect both cytoplasmic and nuclear pools of RRM2B.
• As a polyclonal reagent, it may recognize multiple epitopes; include appropriate controls to confirm specificity in your model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research laboratories, and institutes a comprehensive one-stop platform for IVD raw materials, technical services, and consulting—from concept to clinic. This anti-RRM2B polyclonal antibody is part of our extensive portfolio supporting DNA damage and repair research, enabling reliable assay development. Beyond antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare-target raw-material sourcing to streamline your procurement and accelerate your diagnostic projects.