Anti-RRM2 Rabbit Monoclonal Antibody for WB and ELISA - P31350

Core Product Specifications and Parameters

Biological Background: RRM2 Function and Localization

• RRM2 encodes the small subunit of ribonucleotide reductase (RNR), a heterodimeric enzyme essential for converting ribonucleotides to deoxyribonucleotides, thereby providing the precursors required for DNA synthesis and repair.
• The enzyme complex consists of a large regulatory subunit (RRM1) and this small catalytic subunit (RRM2); dysregulation of RRM2 is associated with cancer progression due to its role in maintaining dNTP pools.
• Beyond deoxynucleotide synthesis, RRM2 has been reported to inhibit Wnt signaling, suggesting a non-canonical function in modulating developmental pathways.
• RRM2 localizes to both the cytoplasm and nucleus, consistent with its roles in dNTP production and potential direct nuclear functions.
• The protein contains a di-iron center and a tyrosyl radical cofactor, both critical for its oxidoreductase activity, and is subject to phosphorylation and ubiquitination, which regulate its stability and activity.
• Alternative splicing of RRM2 produces multiple transcript variants; the canonical isoform has a molecular weight of approximately 45 kDa.
• As a metal-binding phosphoprotein, RRM2 is tightly controlled during the cell cycle, with expression peaking in S phase to support genome duplication.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 10-117) of human RRM2; consider the epitope location when selecting detection antibodies or designing competition assays.
• For Western blot, a band around 45 kDa is expected; validate in lysates from human, mouse, or rat cells, as cross-reactivity with these species is specified.
• In ELISA applications, ensure that the coating antigen and detection reagents are compatible with the rabbit monoclonal antibody format.
• Due to the critical role of RRM2 in DNA synthesis, consider synchronization or cell-cycle enrichment protocols to enhance detection in cycling cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research labs, offering this anti-RRM2 rabbit monoclonal antibody alongside a wide range of IVD raw materials. With expertise in sourcing validated antibody pairs, bulk ancillary reagents, and rare targets like RRM2, we support your projects from concept to clinic. Whether you need specialized reagents for DNA synthesis research or full-scale production materials, CamelBio streamlines your supply chain.