Anti-RRM2 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P31350

Core Product Specifications and Parameters

Biological Background: RRM2 Function and Localization

• RRM2, also known as ribonucleotide reductase small subunit or ribonucleotide reductase small chain, is the small subunit of ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates, providing essential precursors for DNA synthesis.
• It is a key enzyme in the regulation of the deoxyribonucleotide pool and plays a critical role in cell cycle progression and proliferation.
• RRM2 inhibits Wnt signaling, linking nucleotide metabolism to developmental and oncogenic pathways.
• The RRM2 gene belongs to the ribonucleotide reductase family; in humans, the large subunit is encoded by RRM1.
• Subcellular localization is primarily cytoplasmic, but it can also be found in the nucleus.
• Post-translational modifications include phosphorylation and ubiquitin-like conjugation, and the protein requires iron and metal-binding cofactors for its enzymatic activity.
• Alternative splicing generates multiple transcript variants, and the canonical isoform has a calculated molecular weight of approximately 44.9 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the full-length RRM2 (amino acids 1-389), which may recognize multiple isoforms. Validate isoform specificity in the relevant experimental system.
• For Western blotting, a band around 45 kDa is expected under reducing conditions. Optimize the blocking and antibody incubation conditions to minimize background.
• For immunohistochemistry (IHC-P), consider performing antigen retrieval and titrating the antibody to reduce nonspecific staining. A protein blocking step is recommended.
• In immunofluorescence/immunocytochemistry (IF/ICC), confirm potential nuclear and cytoplasmic localization consistent with the biological distribution of RRM2.
• For immunoprecipitation (IP), use appropriate control antibodies and validate binding efficiency.
• The antibody’s cross-reactivity with mouse and rat RRM2 makes it suitable for translational studies in these model organisms, but always verify performance in your specific sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, providing comprehensive access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic development. In nucleotide metabolism and DNA synthesis research, we streamline procurement of high-quality antibodies like this RRM2 polyclonal antibody to accelerate your assay development. Contact our team to discuss tailored sourcing solutions for rare or challenging targets.