Anti-RRM1 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P23921

Core Product Specifications and Parameters

Biological Background: RRM1 Function and Localization

• RRM1 is the large subunit of ribonucleotide reductase (RNR), a heterodimeric enzyme critical for de novo synthesis of deoxyribonucleoside diphosphates (dNDPs) from ribonucleoside diphosphates (rNDPs), providing the monomeric precursors required for DNA replication and repair.
• It functions as an allosteric enzyme, with activity regulated by ATP binding and nucleotide substrate specificity controlled by dATP, dGTP, or dTTP binding to the specificity site.
• RRM1 must complex with a small subunit (RRM2 or p53R2) to form the active holoenzyme; the large subunit contributes the catalytic and allosteric regulatory sites.
• Subcellularly, RRM1 is primarily cytoplasmic, where nucleotide metabolism and DNA precursor synthesis occur.
• Post-translational modifications include acetylation and phosphorylation, which may modulate enzyme activity, stability, or protein-protein interactions.
• Mutations in RRM1 are associated with autosomal recessive mitochondrial DNA depletion syndromes, including progressive external ophthalmoplegia, highlighting its essential role in maintaining balanced dNTP pools for mitochondrial DNA synthesis.
• The protein belongs to the ribonucleotide reductase class I family and shares structural homology with bacterial and eukaryotic RNR large subunits.

Experimental Guidance and Technical Tips

• Based on the immunogen (C-terminal region aa 593-792), the antibody is expected to recognize the full-length RRM1 protein (~90kDa) and may detect isoforms or cleavage products; include appropriate molecular weight markers and controls.
• For Western blotting, use optimized loading and transfer conditions for high-molecular-weight targets; consider enhanced chemiluminescence and extended exposure times.
• In immunofluorescence/ICC, expect cytoplasmic localization; fix and permeabilize cells appropriately (e.g., 4% PFA followed by Triton X-100 permeabilization) to ensure antibody access.
• For immunoprecipitation, confirm compatibility with your lysis buffer and elution conditions; run a positive control lysate (e.g., HeLa or HEK293) to verify capture efficiency.
• The antibody shows reactivity with human and mouse; if testing other species, validate cross-reactivity due to potential sequence divergence in the targeted epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents to streamline your diagnostic development from concept to clinic. This anti-RRM1 rabbit polyclonal antibody supports research in nucleotide metabolism, DNA replication fidelity, and mitochondrial disorders. Contact us for custom antibody development, bulk supply, and technical consulting to accelerate your project.