Anti-RRM1 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P23921

Core Product Specifications and Parameters

Biological Background: RRM1 Function and Localization

• Encodes the large subunit of ribonucleotide reductase, a key enzyme required for de novo DNA synthesis.
• Catalyzes the conversion of ribonucleoside diphosphates to their corresponding deoxyribonucleoside diphosphates, providing the building blocks for DNA replication and repair.
• Localizes predominantly to the cytoplasm, where nucleotide biosynthesis occurs.
• Regulated by allosteric effectors and ATP binding, ensuring balanced dNTP pools.
• Subject to post‑translational modifications such as acetylation and phosphorylation, which may modulate its activity.
• Associated with primary mitochondrial disease and progressive external ophthalmoplegia, linking nucleotide metabolism to mitochondrial function.
• Contains conserved disulfide bonds and nucleotide‑binding domains critical for enzymatic activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 593–792); consider this when mapping binding epitopes or detecting truncated forms.
• For Western blot, a prominent band around 90 kDa is expected in human, mouse, and rat samples; validate in your specific cell or tissue lysates.
• In immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), include appropriate controls and optimize antigen retrieval and permeabilization conditions.
• The antibody can be used for immunoprecipitation (IP) and ELISA; each application may require individual optimization of antibody titer and incubation conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated IVD raw‑material partner, offering reliable access to antibodies such as this anti‑RRM1 polyclonal antibody to support research in nucleic acid metabolism, cancer, and mitochondrial disorders. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare‑target raw materials—all designed to streamline your diagnostic development from concept to clinic.