Anti-RRM1 Monoclonal Antibody for WB, ELISA - P23921

Core Product Specifications and Parameters

Biological Background: RRM1 Function and Localization

• RRM1 encodes the large subunit of ribonucleotide reductase, providing the precursors necessary for DNA synthesis by catalyzing the biosynthesis of deoxyribonucleotides from ribonucleotides.
• Also known as ribonucleoside-diphosphate reductase large subunit, it is essential for DNA replication and repair, linking it to cell cycle regulation and cancer.
• The enzyme is an allosteric enzyme, regulated by ATP binding and other effectors to maintain balanced deoxynucleotide pools.
• Subcellular localization is primarily cytoplasmic, where it performs its catalytic function.
• Post-translational modifications include acetylation and phosphorylation, and the protein contains disulfide bonds important for its structure.
• Keywords from UniProt: oxidoreductase, nucleotide-binding, and involvement in primary mitochondrial disease with progressive external ophthalmoplegia, indicating broader cellular roles.

Experimental Guidance and Technical Tips

• The immunogen was designed against residues 691-792 of human RRM1, a region that may be considered for epitope mapping when investigating antibody specificity across species.
• For Western blot (WB), a band around 90kDa is expected; consider using appropriate loading controls and validate in the relevant sample system.
• ELISA application may be employed for quantitative detection; optimization of antibody dilutions and assay conditions is recommended.
• Cross-reactivity with mouse and rat suggests potential utility in rodent model studies; however, confirm sequence homology of the immunogen region in these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For RRM1-targeted research in DNA synthesis and oncology, we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our extensive supply chain can source rare target raw materials, ensuring your project's success with reliable immunoreagents.