Anti-RRBP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9P2E9

Core Product Specifications and Parameters

Biological Background: RRBP1 Function and Localization

• RRBP1 encodes the ribosome-binding protein 1, also known as 180 kDa ribosome receptor homolog (RRp), ES/130-related protein, or ribosome receptor protein.
• The protein acts as a ribosome receptor on the endoplasmic reticulum (ER) membrane, mediating the interaction between ribosomes and the ER, crucial for co-translational protein translocation.
• RRBP1 localizes to the endoplasmic reticulum membrane, indicating its role in the secretory pathway and protein synthesis.
• The gene undergoes alternative splicing, producing multiple transcript variants, which may result in functionally distinct isoforms.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, suggesting regulatory mechanisms for protein stability and activity.
• The predicted molecular weight is approximately 152 kDa, consistent with its designation as p180.
• The protein contains a transmembrane domain and repeats, aligning with its membrane-bound receptor function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-140) of human RRBP1. Pre-absorption controls may be considered to confirm specificity.
• For Western blotting (WB), the predicted molecular weight is ~152 kDa; however, alternative splicing or post-translational modifications may result in multiple bands. Optimize gel percentage and transfer conditions.
• For immunofluorescence/immunocytochemistry (IF/ICC), fixation and permeabilization conditions may need optimization due to ER membrane localization; detergent extraction could enhance signal.
• For ELISA, ensure coating efficiency of the target antigen; pairing with a detection antibody may require validation.
• Cross-reactivity with mouse is reported; this antibody can be used in mouse models, but species-specific variations should be validated.
• Positive controls from human or mouse tissues expressing RRBP1 (e.g., secretory cells) could be useful.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, including this anti-RRBP1 polyclonal antibody, to support your research in endoplasmic reticulum and protein translocation studies. With validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, we empower diagnostic manufacturers to accelerate development from concept to clinic.