Anti-RRAS2 Rabbit Polyclonal Antibody for WB, ELISA - P62070

Core Product Specifications and Parameters

Biological Background: RRAS2 Function and Localization

• RRAS2, also known as Ras-like protein TC21 or teratocarcinoma oncogene, is a member of the Ras superfamily of small GTPases encoded by the RRAS2 gene. Alternative splicing produces multiple isoforms.
• It functions as a GTP-binding protein with intrinsic GTPase activity, playing a central role in the regulation of the MAPK signaling pathway and thereby controlling diverse cellular processes. Related references: PMID:31130282 PMID:31130285 PMID:39809765
• RRAS2 is critically involved in craniofacial development, as demonstrated by genetic studies linking its function to morphogenetic events during embryogenesis. Related references: PMID:31130282 PMID:31130285
• At the subcellular level, RRAS2 localizes primarily to the cell membrane and the Golgi apparatus membrane, consistent with its roles in signal transduction and vesicular trafficking.
• It is ubiquitously expressed across human tissues, with the highest levels detected in heart, placenta, and skeletal muscle, moderate expression in lung and liver, and lower levels in brain, kidney, and pancreas.
• Post-translational modifications of RRAS2 include palmitoylation, prenylation, phosphorylation, acetylation, and methylation, which modulate its membrane association, activity, and interactions.
• Classified as a proto-oncogene, aberrant activation or overexpression of RRAS2 has been implicated in oncogenic transformation and is associated with disease variants in certain cancers.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human RRAS2 (amino acids 1-204), which may confer broad isoform reactivity; consider verifying specificity in your experimental system.
• For Western blot, the predicted molecular weight is approximately 23 kDa; use appropriate gel percentages and membrane transfer conditions for low-molecular-weight proteins.
• As a polyclonal antibody, lot-to-lot consistency may vary; include a positive control (e.g., HEK293 or HeLa lysates) and validate performance before critical experiments.
• ELISA applications may require optimizing coating antigen concentration and antibody dilution; recombinant protein immunogen can serve as a positive control.

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CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies for Ras signaling and cancer research. We supply optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing services to streamline your development pipeline. Whether you need consistent antibody pairs for kit assembly or hard-to-find raw materials, CamelBio offers reliable solutions tailored to your project requirements.