Anti-RRAGD Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9NQL2

Core Product Specifications and Parameters

Biological Background: RRAGD Function and Localization

• Ras-related GTP-binding protein D (RRAGD) is a member of the Ras superfamily of small GTPases, functioning as a heterodimer with RagA/RRAGA or RagB/RRAGB to mediate amino acid sensing.
• In its active GDP-bound form, RRAGD partners with GTP-bound RagA/B to recruit mTORC1 to the lysosomal surface, enabling activation by RHEB and orchestrating cellular responses to amino acid availability (PubMed:20381137, PubMed:24095279, PubMed:34607910). Related references: PMID:20381137, PMID:24095279, PMID:34607910
• The protein cycles between an inactive GTP-bound state and an active GDP-bound state; the nucleotide status of RRAGD is key to determining mTORC1 localization and activity (PubMed:24095279). Related references: PMID:24095279
• RRAGD also participates in a non-canonical mTORC1 pathway, independent of RHEB, where GDP-bound RRAGD recruits MiT/TFE transcription factors TFEB and TFE3 for phosphorylation (PubMed:32612235). Related references: PMID:32612235
• Subcellularly, RRAGD localizes to the cytoplasm and nucleus, and translocates to the lysosome membrane upon amino acid stimulation, facilitating spatial control of mTORC1 signaling.
• Disease-associated variants of RRAGD have been linked to cardiomyopathy and primary hypomagnesemia, highlighting its clinical significance.

Experimental Guidance and Technical Tips

• For western blot (WB), consider using standard protocols with a dilution optimized for a ~46kDa band; validate in whole-cell lysates from human, mouse, or rat tissues or cell lines.
• In immunohistochemistry (IHC-P), antigen retrieval methods may be required; include appropriate controls for tissue-specific expression.
• ELISA applications may benefit from pairing this antibody with a detection system tailored for rabbit polyclonal antibodies; confirm linearity and sensitivity in the relevant sample matrix.
• Given RRAGD’s role in mTORC1 regulation, this antibody is a valuable tool for studying amino acid signaling, autophagy, and metabolic pathways.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw material sourcing, offering validated polyclonal and monoclonal antibodies, antigen-grade recombinant proteins, and ancillary reagents. This anti-RRAGD polyclonal antibody supports research into mTORC1 signaling and related metabolic disorders, and our supply chain can accommodate bulk orders or custom development projects. Whether you need this product or other critical raw materials for diagnostic assay development, CamelBio streamlines procurement from concept to clinic.