Anti-RRAGA Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q7L523

Core Product Specifications and Parameters

Biological Background: Ras-related GTP-binding protein A (RRAGA) Function and Localization

• RRAGA (Ras-related GTP-binding protein A), also known as FIP-1, is a member of the Ras superfamily of small GTPases.
• It plays a central role in amino acid sensing by regulating the mTORC1 signaling pathway, acting as a heterodimer with RagC or RagD, and cycling between active GTP-bound and inactive GDP-bound forms. Related references: PMID:20381137 PMID:24095279 PMID:25936802
• In its GTP-bound active form, RRAGA recruits mTORC1 to the lysosomal membrane, where it is activated by RHEB, thereby coupling nutrient availability to cell growth. Related references: PMID:20381137 PMID:25936802 PMID:31601708
• RRAGA is also involved in the RCC1/Ran-GTPase pathway and may mediate TNF-induced cell death. Related references: PMID:9394008 PMID:8995684
• The protein contains a GTP-binding domain and is annotated as a phosphoprotein and a target for ubiquitin-like conjugation, suggesting regulation by post-translational modifications.
• Subcellularly, RRAGA localizes to the cytoplasm and nucleus, and associates with the lysosome membrane upon activation.
• It is ubiquitously expressed, with highest levels in skeletal muscle, heart, and brain, consistent with its role in nutrient sensing in metabolically active tissues.
• Additionally, RRAGA is implicated in host-virus interactions, serving as a cellular target for adenovirus E3-14.7K, which may affect cell death pathways.

Experimental Guidance and Technical Tips

• For Western blotting, a specific band at approximately 37 kDa is anticipated; consider optimizing blocking conditions and primary antibody dilution to minimize background.
• In immunofluorescence, fixation and permeabilization protocols may need adjustment to preserve lysosomal membrane association and improve detection of the endogenous protein.
• The immunogen corresponds to a C-terminal peptide (aa 213–313), suggesting the antibody may recognize both GDP- and GTP-bound conformations; this can be leveraged in assays monitoring activation state.
• For ELISA, pair with a suitable capture/detection antibody and validate linearity and sensitivity in the relevant sample matrix.

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