Anti-RRAGA Polyclonal Antibody for WB, IHC-P, ELISA - Q7L523

Core Product Specifications and Parameters

Biological Background: RRAGA Function and Localization

• RRAGA (Ras-related GTP-binding protein A), also known as FIP-1 or RAGA, is a member of the Rag GTPase family.
• Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. RRAGA forms heterodimeric Rag complexes with RagC/RRAGC or RagD/RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. Related references: PMID:20381137, PMID:24095279, PMID:25936802
• In its GTP-bound active form, RRAGA promotes the recruitment of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Related references: PMID:31601708
• May play a direct role in a TNF signaling pathway leading to induction of cell death. Related references: PMID:8995684
• Involved in the RCC1/Ran-GTPase pathway. Related references: PMID:9394008
• Subcellular localization includes the cytoplasm, nucleus, and lysosome membrane.
• Ubiquitously expressed, with highest levels of expression in skeletal muscle, heart, and brain.
• Keywords: GTP-binding, hydrolase, apoptosis, host-virus interaction, isopeptide bond, phosphoprotein, reference proteome.
• (Microbial infection) May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF functions, thereby affecting cell death.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 213–313 of human RRAGA. Consider that the antibody may recognize the full-length protein as well as potential isoforms.
• For Western Blot, the predicted molecular weight is approximately 37 kDa, though UniProt annotates 36.6 kDa. Validate in the relevant sample system and consider loading controls.
• For IHC-P, tissue sections from skeletal muscle, heart, or brain are recommended for positive staining based on expression data. Optimization of antigen retrieval and antibody dilution is advised.
• For ELISA, the immunizing peptide or recombinant protein may serve as a positive control.
• Always titrate the antibody for optimal performance in your specific application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, technical services, and consulting—from concept to clinic. For studies on mTORC1 signaling or amino acid sensing, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our one-stop model streamlines procurement, supporting your RRAGA-targeted projects with reliable supply and rare-target sourcing capabilities.