Anti-RPS9 Rabbit Polyclonal Antibody for WB and ELISA - P46781

Core Product Specifications and Parameters

Biological Background: RPS9 Function and Localization

• RPS9 encodes the 40S ribosomal protein S9, also known as small ribosomal subunit protein uS4, a core component of the 40S small ribosomal subunit.
• As a ribosomal protein, RPS9 is essential for translation, participating in the small subunit that binds mRNA and decodes genetic information (PubMed:23636399). Related references: PMID:23636399
• During ribosome biogenesis, RPS9 is part of the small subunit (SSU) processome in the nucleolus, where it assists in pre-rRNA folding, modification, and cleavage, facilitating maturation of the 18S rRNA (PubMed:34516797). Related references: PMID:34516797
• Subcellularly, RPS9 is found in the cytoplasm as part of mature ribosomes, but also localizes to the nucleus and nucleolus during ribosome assembly.
• The protein undergoes multiple post-translational modifications, including acetylation, phosphorylation, and isopeptide bond formation, which may modulate its assembly and function.
• RPS9 is evolutionarily conserved, with the human protein showing high sequence homology to mouse and rat orthologs, consistent with the observed cross-reactivity of this antibody.
• UniProt keywords associated with RPS9 include: 3D-structure, direct protein sequencing, phosphoprotein, RNA-binding, and ribonucleoprotein.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a recombinant protein covering amino acids 1–162 of human RPS9. This N-terminal region may be accessible, making the antibody suitable for detecting both nascent and mature RPS9.
• For Western blot, a band at approximately 23 kDa is expected; consider using a positive control such as HeLa or HEK293 cell lysate.
• In ELISA, immobilized recombinant RPS9 or peptide may be used; titrate antibody concentration to optimize signal.
• Given the high sequence identity among human, mouse, and rat, cross-reactivity is anticipated, but performance should be verified in each species' sample matrix.
• As RPS9 is a housekeeping protein, expression is generally constitutive; loading controls such as β-actin or GAPDH are recommended for normalization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to streamline sourcing of IVD raw materials, including validated antibodies for ribosomal and translational targets like RPS9. From bulk polyclonal antibodies and matched antibody pairs to ancillary reagents, we provide reliable supply solutions tailored to your assay development needs. Whether you require routine restocks or rare-target raw-material sourcing, CamelBio helps bridge the gap from concept to clinic.