Anti-RPS8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P62241

Core Product Specifications and Parameters

Biological Background: RPS8 Function and Localization

• RPS8, also known as 40S ribosomal protein S8 or eS8, is a core protein of the small (40S) ribosomal subunit essential for protein synthesis (PubMed:23636399). Related references: PMID:23636399

• During ribosomal assembly, RPS8 incorporates into the small subunit (SSU) processome within the nucleolus, facilitating pre-rRNA processing, modifications, and maturation (PubMed:34516797). Related references: PMID:34516797

• RPS8 localizes to multiple subcellular compartments including the cytoplasm, membrane, nucleus, and nucleolus, reflecting its dynamic roles in translation and ribosome biogenesis.

• Post-translational modifications of RPS8 include acetylation, phosphorylation, myristoylation, and isopeptide bond formation, which may regulate its stability, localization, or interactions.

• As part of the ribosome, RPS8 is involved in decoding mRNA and ensuring translational accuracy, a fundamental process in all living cells.

• The protein is subject to ubiquitin conjugation (Ubl conjugation), potentially targeting it for proteasomal degradation under certain conditions.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 10-100 of human RPS8, a region that is highly conserved across mammals; therefore, the antibody is expected to cross-react with mouse and rat RPS8 as indicated.
• For Western blot (WB), expect a specific band at approximately 24 kDa; include appropriate loading controls and consider validating with relevant cell or tissue lysates.
• When performing immunofluorescence (IF/ICC), note that RPS8 predominantly localizes to the cytoplasm and nucleolus, so permeabilization is required for nucleolar detection; optimize fixation and permeabilization conditions for your sample.
• For ELISA applications, pair with an appropriate detection antibody and validate in the relevant sample matrix, accounting for potential matrix effects.
• As with all polyclonal antibodies, lot-to-lot variability may exist; it is advisable to confirm specificity by knock-down or knock-out experiments in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material procurement, offering antibodies like this RPS8 polyclonal antibody essential for ribosomal protein studies and translation research. Through our validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, we streamline your development pipeline. Our expertise also extends to sourcing rare targets, ensuring you have reliable access to the materials needed from concept to clinic.