Anti-RPS8 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - P62241

Core Product Specifications and Parameters

Biological Background: RPS8 Function and Localization

• RPS8 is a component of the small ribosomal subunit and is essential for cellular protein synthesis. Related references: PMID:23636399
• As part of the small subunit (SSU) processome, RPS8 participates in the assembly and maturation of the small eukaryotic ribosomal subunit, involving RNA folding, modifications, and pre-rRNA processing. Related references: PMID:34516797
• The protein localizes to the cytoplasm, and has also been detected in the membrane, nucleus, and nucleolus.
• RPS8 belongs to the eukaryotic ribosomal protein eS8 family and is a key component of ribonucleoprotein complexes.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation, as identified by proteomic analyses.
• It may play a role in nucleolar function and ribosome biogenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human RPS8 protein (amino acids 1-208). This recombinant antigen should ensure recognition of the native target, but validation in the relevant sample system is recommended.
• For Western blot, a band at approximately 24 kDa is expected; always include appropriate molecular weight markers.
• For immunofluorescence/immunocytochemistry, consider that RPS8 localizes to both the cytoplasm and nucleus; optimize fixation and permeabilization protocols.
• For immunoprecipitation, test the antibody performance under native or crosslinking conditions as needed.
• For ELISA, the recombinant fusion protein may serve as a positive control; determine optimal coating concentrations.
• Cross-reactivity with mouse and rat has been reported; validate performance in the species of interest.

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