Anti-RPS6KL1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y6S9

Core Product Specifications and Parameters

Biological Background: RPS6KL1 Function and Localization

• RPS6KL1 (Ribosomal protein S6 kinase-like 1), also known as RSKL2, is a putative serine/threonine-protein kinase belonging to the protein kinase superfamily.
• The protein contains an ATP-binding site and a nucleotide-binding domain, characteristic of kinases that transfer phosphate groups from ATP to target substrates.
• As a ribosomal protein, RPS6KL1 is predicted to be a component of the ribosome, possibly involved in ribosomal biogenesis or translation regulation.
• The gene undergoes alternative splicing, potentially generating multiple protein isoforms with distinct functions or regulatory roles.
• Identified by proteomics approaches, the protein is part of the reference proteome for Homo sapiens.
• Subcellular localization is not fully characterized but may be associated with the ribosome in the cytoplasm or nucleus.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 60 kDa; include appropriate positive controls (e.g., human, mouse, or rat cell lysates) and validate band specificity using a blocking peptide or knockdown approach.
• For immunofluorescence/ICC, optimize fixation and permeabilization conditions; consider testing both Triton X-100 and saponin-based permeabilization to determine the optimal subcellular staining pattern.
• In ELISA applications, use the antibody as a capture or detection reagent after validating sensitivity against recombinant RPS6KL1 protein.
• Given the cross-reactivity with mouse and rat, the antibody can be used in comparative or translational studies involving these species, but always confirm species-specific reactivity in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Researchers investigating ribosomal biology and kinase signaling pathways can leverage CamelBio’s expertise for antibody selection and rare-target raw-material procurement, accelerating diagnostic R&D from concept to clinic.