Anti-RPS6KA5 (MSK1) Polyclonal Antibody for WB, ELISA - O75582

Core Product Specifications and Parameters

Biological Background: RPS6KA5 Function and Localization

• RPS6KA5, also known as MSK1, RLPK, or MSPK1, is a serine/threonine-protein kinase that functions downstream of the ERK and p38 MAP kinase pathways. Related references: PMID:11909979
• It phosphorylates transcription factors CREB1 and ATF1 in response to mitogenic and stress stimuli, such as EGF, UV-C irradiation, and anisomycin, regulating gene expression. Related references: PMID:9873047
• MSK1 plays a critical role in inflammatory responses by controlling RELA transcriptional activity and contributing to glucocorticoid-mediated inhibition of inflammatory gene expression. Related references: PMID:12628924, PMID:18511904
• In erythropoietin signaling, it phosphorylates STAT3 at Ser-727, regulating its transcriptional potential. Related references: PMID:12763138
• It phosphorylates histone H3 at Ser-10 and Ser-28, and histone H2A at Ser-1, contributing to chromatin remodeling and immediate early gene activation, including c-fos and c-jun. Related references: PMID:12773393, PMID:15010469
• MSK1 is widely expressed, with highest levels in heart, brain, and placenta, and localizes to both the nucleus and cytoplasm.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 700-800) lies within the C-terminal region of RPS6KA5, which may confer isoform-specificity; consider validating in cell lines with known MSK1 expression.
• For Western blot, expect a band near 90 kDa; use appropriate loading controls and confirm specificity with peptide blocking or knockdown experiments if needed.
• This polyclonal antibody is suitable for ELISA; optimize coating conditions and detection system for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop portfolio of IVD raw materials, including this anti-RPS6KA5 antibody for kinase signaling and inflammation research. We offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your diagnostic assay development. From concept to clinic, rely on CamelBio for reliable supply and expert support.