Anti-RPS6KA4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75676

Core Product Specifications and Parameters

Biological Background: RPS6KA4 (MSK2) Function and Localization

• Protein identity: RPS6KA4, also known as MSK2, RSK-B, or S6K-alpha-4, is an 86-kDa serine/threonine-protein kinase belonging to the RSK family. Encoded by the RPS6KA4 gene (GeneID: 8986), alternative splicing generates multiple isoforms.
• Core kinase function: It phosphorylates transcription factors CREB1 and ATF1 in response to mitogenic (e.g., EGF) and stress stimuli (e.g., UV-C, anisomycin), thereby regulating immediate early gene expression.
• Histone modification: MSK2 phosphorylates histone H3 at Ser-10 (and possibly Ser-28), leading to chromatin relaxation and transcriptional activation of genes like c-fos and c-jun. It also phosphorylates HMGN1 (HMG14), further modulating chromatin structure.
• Inflammatory signaling: In macrophages, MSK2 acts downstream of TLR4 to limit pro-inflammatory cytokine production by inducing DUSP1 (MAP kinase phosphatase) and IL10 via CREB1/ATF1, highlighting its anti-inflammatory role.
• RELA regulation: MSK2 is essential for TNF-induced RELA (NF-κB p65) transcriptional activity, underpinning its role in NF-κB signaling and inflammation.
• Subcellular localization: Primarily nuclear, consistent with its function in transcription factor and histone phosphorylation. Associated keywords include nucleus, kinase, inflammatory response, and stress response.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 673-772), ensuring recognition of full-length RPS6KA4. Validate specificity by peptide competition or knockdown experiments in the relevant sample system.
• For Western blot, the predicted molecular weight is ~86 kDa. Use appropriate positive (e.g., HeLa, HEK293) and negative controls, and consider phosphatase inhibitors to preserve phosphorylation-dependent epitopes.
• For IHC-P, optimize antigen retrieval and antibody dilution. Validate staining in human or mouse tissues known to express MSK2 (e.g., immune cells, high-turnover tissues).
• For ELISA, the peptide immunogen supports direct or sandwich ELISA formats. Ensure cross-reactivity testing if using mouse samples.
• As a polyclonal antibody, expect some lot-to-lot variability. Consider functional validation in your specific assay context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop IVD raw-material sourcing partner, supporting diagnostic manufacturers and research labs worldwide. This anti-RPS6KA4 (MSK2) rabbit polyclonal antibody is ideal for studies on stress and inflammatory signaling pathways. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonals/polyclonals, bulk ancillary reagents, and rare target sourcing to streamline your assay development from concept to clinic.