Anti-RPS6KA3 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P51812

Core Product Specifications and Parameters

Biological Background: RPS6KA3 Function and Localization

• RPS6KA3 (RSK2) is a serine/threonine-protein kinase acting downstream of ERK/MAPK signaling to mediate mitogenic and stress-induced responses, regulating transcription, translation, and cell survival. Related references: PMID:16213824, PMID:16223362, PMID:17360704
• Upon EGF stimulation, it phosphorylates CREB1 and histone H3 at Ser10, promoting immediate-early gene transcription. Related references: PMID:10436156, PMID:9770464
• Phosphorylates and activates transcription factors NR4A1/NUR77, ETV1/ER81, and the cofactor CREBBP. Related references: PMID:16223362
• Regulates translation by phosphorylating RPS6 (mTOR-independent) and EIF4B to facilitate cap-dependent initiation. Related references: PMID:17360704, PMID:18508509, PMID:18813292
• Integrates mTOR nutrient sensing via phosphorylation of TSC2 and RPTOR, modulating mTORC1 activity. Related references: PMID:18722121
• Promotes cell survival by phosphorylating and inhibiting pro-apoptotic BAD and DAPK1. Related references: PMID:16213824
• Regulates cell migration through EPHA2 phosphorylation at Ser897. Related references: PMID:26158630
• Localizes to both nucleus and cytoplasm; highest expression in skeletal muscle. Mutations are associated with X-linked intellectual disability (MRX19, XLID19).

Experimental Guidance and Technical Tips

• The immunogen spans residues 68–327 within the N-terminal kinase domain; consider this region when assessing phospho-specificity or potential cross-reactivity with related kinases.
• For Western blot, an ~84 kDa band is expected. Validate detection in relevant cell lines (e.g., HeLa, HEK293) stimulated with EGF or serum to observe activation-dependent changes.
• When performing IHC-P, optimize antigen retrieval and fixation conditions; high expression in skeletal muscle may serve as a positive control.
• This polyclonal antibody recognizes human, mouse, and rat RPS6KA3; verify reactivity in your specific model system.
• As a rabbit polyclonal, batch-to-batch consistency is typical; include appropriate controls and normalization for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this RPS6KA3 rabbit polyclonal antibody as part of a comprehensive IVD raw material portfolio for diagnostic assay development. Our one-stop sourcing platform supports researchers studying the MAPK/ERK signaling pathway and cell proliferation, providing validated antibody pairs, recombinant antigens, and ancillary reagents. Contact us for customized solutions or assistance with rare-target sourcing.