Anti-RPS6KA2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15349

Core Product Specifications and Parameters

Biological Background: RPS6KA2 Function and Localization

• RPS6KA2 encodes the ribosomal protein S6 kinase alpha-2, also known as RSK3, p90RSK2, MAPKAPK1C, and S6K-alpha2, a member of the p90 ribosomal S6 kinase (RSK) family.
• It is a serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling, mediating mitogenic and stress-induced activation of transcription factors.
• Plays a role in regulating translation, cellular proliferation, survival, and differentiation.
• May function as a tumor suppressor in epithelial ovarian cancer cells, highlighting its potential involvement in cancer biology.
• Localizes to both the nucleus and cytoplasm, consistent with its roles in signal transduction and gene expression regulation.
• Contains two kinase domains, as indicated by the presence of ATP-binding and nucleotide-binding motifs, and is classified as a transferase.
• Key post-translational modifications include phosphorylation, and the protein binds magnesium as a cofactor.
• Widely expressed with higher levels in lung, skeletal muscle, brain, uterus, ovary, thyroid, and prostate.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 632–733 near the C-terminus of human RPS6KA2. When designing detection reagents, consider epitope accessibility and pairing with antibodies targeting distinct regions.
• For Western blotting, a band around 83 kDa is expected; validate the antibody using positive control lysates from tissues with known RPS6KA2 expression, such as lung or ovary.
• In immunohistochemistry and immunocytochemistry, nuclear and cytoplasmic staining may be observed; optimize fixation and permeabilization conditions for your sample type.
• Cross-reactivity with mouse and rat orthologs is reported; verify reactivity in your species of interest using appropriate controls.
• For ELISA applications, pair with a matched detection antibody or use in sandwich formats as per your assay requirements; confirm specificity with recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, offering validated polyclonal and monoclonal antibodies, antigen-grade recombinant proteins, and ancillary reagents to support diagnostic kit development. The RPS6KA2 antibody serves as a tool for investigating MAPK/ERK signaling and tumor suppression mechanisms, and we can assist with custom antibody generation, bulk supply, and rare-target sourcing to streamline your assay development from concept to clinic.