Anti-RPS5 Rabbit Polyclonal Antibody for WB and ELISA - P46782

Core Product Specifications and Parameters

Biological Background: RPS5 Function and Localization

• RPS5, also known as 40S ribosomal protein S5 or uS7, is a component of the small ribosomal subunit, a large ribonucleoprotein complex that catalyzes protein synthesis in the cell.
  Related references:
  PMID:23636399
• RPS5 participates in early ribosome assembly as part of the small subunit (SSU) processome, interacting with pre-rRNA and biogenesis factors to facilitate RNA folding, modification, and cleavage.
  Related references:
  PMID:34516797
• RPS5 localizes primarily to the cytoplasm but is also found in the nucleus and nucleolus, reflecting its dual roles in mature ribosome function and ribosome biogenesis.
• Post-translational modifications on RPS5 include acetylation and phosphorylation; it can also be conjugated to ubiquitin-like proteins via isopeptide bonds.
• RPS5 is a conserved ribosomal protein identified by proteomics and direct protein sequencing, serving as a reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human RPS5 sequence (amino acids 1–204), ensuring broad epitope coverage. For Western blotting, a major band around 23 kDa is expected; include lysates from human, mouse, or rat cells as positive controls.
• For ELISA applications, it is advisable to perform a titration series to determine the optimal antibody coating concentration and detection conditions.
• Although this antibody cross-reacts with mouse and rat RPS5, cross-reactivity with other species should be independently validated in the desired experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a reliable supply of validated IVD raw materials, including polyclonal antibodies like this anti-RPS5 antibody. Our one-stop platform supports your workflow with bulk ancillary reagents, optimized antibody pairs, and rare target sourcing capabilities, ensuring you can focus on ribosome biology and translational research applications without supply chain interruptions.