Anti-RPS4X Rabbit Polyclonal Antibody for WB and ELISA - P62701

Core Product Specifications and Parameters

Biological Background: RPS4X Function and Localization

• RPS4X encodes the small ribosomal subunit protein eS4, X isoform, a core component of the 40S ribosomal subunit essential for cellular protein synthesis. Related references: PMID:23636399
• During ribosome assembly, eS4 participates in the small subunit (SSU) processome, facilitating pre-rRNA folding, modification, and cleavage in the nucleolus. Related references: PMID:34516797
• eS4 localizes predominantly in the cytoplasm, but is also found in the nucleus and nucleolus, reflecting its dual role in ribosome function and biogenesis.
• Post-translational modifications include acetylation and ubiquitin-like conjugation, which may regulate its ribosomal integration and stability.
• The protein is highly conserved across human, mouse, and rat, with a predicted molecular weight of approximately 30 kDa.
• Alternative names include S4, CCG2, SCAR, SCR10, and DXS306, reflecting its identification in various cellular contexts.

Experimental Guidance and Technical Tips

• For Western blot analysis, the target band is expected around 30 kDa; consider using a loading control of similar molecular weight and validate specificity with appropriate cell lysates.
• Due to its polyclonal nature, empirical optimization of antibody dilution is recommended for both WB and ELISA, and signal specificity should be confirmed using a negative control such as knockout or knockdown samples.
• Given the high cross-reactivity with mouse and rat, this antibody is suitable for comparative studies across species, but the epitope conservation should be independently verified in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, technical services, and consulting. This anti-RPS4X antibody is part of our extensive portfolio for ribosomal protein and translation research. We offer validated antibody pairs, bulk ancillary reagents, and tailored sourcing solutions for rare and emerging targets to support your assay development from concept to clinic.