Anti-RPS3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P23396

Core Product Specifications and Parameters

Biological Background: RPS3 Function and Localization

• Component of the 40S small ribosomal subunit, essential for cytoplasmic protein synthesis.
  Related references:
  PMID:23636399 PMID:8706699
• Possesses endonuclease activity and participates in DNA repair by cleaving phosphodiester bonds of DNAs containing altered bases, with a preference for supercoiled DNA.
  Related references:
  PMID:7775413 PMID:15707971
• Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common oxidative DNA lesion, and stimulates the N-glycosylase activity of the base excision repair protein OGG1.
  Related references:
  PMID:14706345 PMID:15518571
• Enhances uracil excision by UNG1 and stimulates APEX1-mediated phosphodiester backbone cleavage, facilitating base excision repair.
  Related references:
  PMID:18973764
• Localizes to mitochondria where it reduces cellular reactive oxygen species (ROS) levels and mitochondrial DNA damage. Also negatively regulates DNA repair upon hydrogen peroxide exposure.
  Related references:
  PMID:23911537 PMID:17049931
• Acts as a transcriptional regulator by associating with NF-kappa-B p65-p50 complex, enhancing DNA binding and promoting target gene transcription. Represses its own translation by binding its cognate mRNA.
  Related references:
  PMID:18045535 PMID:20217897
• Protects TP53/p53 from MDM2-mediated ubiquitination and degradation, contributing to genome stability.
  Related references:
  PMID:19656744
• Involved in mitotic spindle formation and chromosome movement by regulating microtubule polymerization, and induces apoptosis through CASP8 activation and synergism with E2F1.
  Related references:
  PMID:23131551 PMID:14988002 PMID:20605787

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-243) of human RPS3, which may recognize full-length endogenous protein and potential isoforms; verify specificity in your model system.
• For Western blotting, a band at approximately 27 kDa is expected; consider using whole cell lysates from human, mouse, or rat cells as positive controls.
• Due to RPS3’s localization in both cytoplasm and nucleus, immunofluorescence and immunohistochemistry may reveal diffuse cytoplasmic and nucleolar staining; validate with DAPI co-staining and appropriate subcellular markers.
• As a polyclonal antibody, optimal working dilutions should be empirically determined for each application and sample type.
• For ELISA applications, use recombinant RPS3 protein or peptide as coating antigen to assess antibody titer and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized mono- and polyclonal antibodies, and bulk ancillary reagents. For ribosome research, DNA repair, or apoptosis studies, our one-stop sourcing platform streamlines procurement of high-quality antibodies like RPS3 polyclonal antibody, alongside rare-target raw materials and technical consultation. Simplify your supply chain from concept to clinic with CamelBio.