Anti-RPS3 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P23396

Core Product Specifications and Parameters

Biological Background: RPS3 Function and Localization

• RPS3 (uS3, 40S ribosomal protein S3) is a component of the small ribosomal subunit, vital for cytoplasmic protein synthesis. Related references: PMID:23636399, PMID:8706699

• It localizes to the cytoplasm, nucleus, nucleolus, mitochondrial inner membrane, and mitotic spindle, reflecting its diverse roles.

• RPS3 displays endonuclease activity and preferentially binds to DNA containing 8-oxoguanine lesions, implicating it in DNA damage recognition. Related references: PMID:7775413, PMID:15707971, PMID:14706345, PMID:18610840

• It stimulates the base excision repair pathway by enhancing the activities of OGG1, UNG1, and APEX1. Related references: PMID:15518571, PMID:18973764

• In mitochondria, RPS3 reduces cellular ROS levels and limits mitochondrial DNA damage, though it can also downregulate DNA repair upon hydrogen peroxide exposure. Related references: PMID:23911537, PMID:17049931

• RPS3 participates in transcriptional regulation by binding RELA/p65 within the NF-kappa-B complex, promoting target gene expression. Related references: PMID:18045535

• It is involved in apoptosis through CASP8 activation, synergistic pro-apoptotic gene upregulation with E2F1, and TRADD-mediated JNK activation upon UV stress. Related references: PMID:14988002, PMID:20605787, PMID:22510408

• Additionally, RPS3 represses its own translation, protects p53 from MDM2-mediated ubiquitination, and contributes to mitotic spindle formation and chromosome movement. Related references: PMID:20217897, PMID:19656744, PMID:23131551

Experimental Guidance and Technical Tips

• For Western blotting, consider using a 10% SDS-PAGE gel and transfer to PVDF membrane overnight; expect a band at approximately 27 kDa.
• For immunofluorescence, validate in fixed and permeabilized cells, as RPS3 localizes to multiple subcellular compartments (nucleus, cytoplasm, mitochondria).
• For ELISA, the antibody may be suitable for detection of recombinant or endogenous RPS3; optimize coating and blocking conditions.
• The immunogen sequence (aa 100-200) is conserved among human, mouse, and rat, supporting the observed cross-reactivity; confirm for other species before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-RPS3 antibody. Our portfolio spans ribosomal proteins and DNA repair targets, supported by bulk reagent supply and technical consultation from concept to clinic. Whether you need antibody pairs, ancillary reagents, or rare target sourcing, CamelBio is your reliable partner for accelerating assay development.