Anti-RPS3 Polyclonal Antibody for WB, IF/ICC, ELISA - P23396

Core Product Specifications and Parameters

Biological Background: RPS3 Function and Localization

• Component of the small ribosomal subunit, essential for protein synthesis. Related references: PMID:23636399, PMID:8706699
• Possesses endonuclease activity and plays a role in DNA repair, cleaving damaged DNA and showing high affinity for 8-oxoG lesions. Related references: PMID:7775413, PMID:15707971, PMID:14706345, PMID:18610840
• Stimulates base excision repair enzymes OGG1, UNG1, and APEX1, enhancing DNA damage removal. Related references: PMID:15518571, PMID:18973764
• Modulates transcription by binding to RELA/p65 subunit of NF-kappa-B, enhancing DNA binding and target gene expression. Related references: PMID:18045535
• In mitochondria, reduces ROS levels and mitochondrial DNA damage; also negatively regulates DNA repair under oxidative stress. Related references: PMID:23911537, PMID:17049931
• Regulates apoptosis: activates CASP8, induces neuronal apoptosis via E2F1/BIM/HRK, and interacts with TRADD for JNK activation. Related references: PMID:14988002, PMID:20605787, PMID:22510408
• Involved in mitosis and spindle formation by regulating microtubule polymerization. Related references: PMID:23131551
• Subcellular localization includes cytoplasm, nucleus (nucleolus), mitochondrion inner membrane, and the mitotic spindle. Subject to post-translational modifications such as acetylation, methylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1–243) of human RPS3; this sequence is conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blotting, expect a band around 27 kDa. Be aware that alternative splicing or post-translational modifications may produce additional bands.
• For immunofluorescence/ICC, consider the protein’s multiple subcellular localizations (cytoplasm, nucleus, mitochondria, spindle) and validate staining patterns in your model system.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; it is advisable to include positive and negative controls for each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated polyclonal antibodies for ribosomal targets like RPS3. Our portfolio spans antibody pairs, optimized monoclonals/polyclonals, and bulk ancillary reagents—supporting your projects from early development to clinical kit production. Whether you need rare-target sourcing or scalable supply, CamelBio simplifies your raw-material procurement.