Anti-RPS28 Polyclonal Antibody for WB, IF/ICC, ELISA - P62857

Core Product Specifications and Parameters

Biological Background: RPS28 Function and Localization

• RPS28 is a component of the small ribosomal subunit (40S), essential for protein synthesis. The ribosome is a large ribonucleoprotein complex responsible for translating mRNA into proteins. Related references: PMID:23636399, PMID:25901680, PMID:25957688
• It is part of the small subunit (SSU) processome, the first precursor of the small eukaryotic ribosomal subunit, involved in ribosome biogenesis. During SSU assembly, RPS28 associates with pre-rRNA and contributes to RNA folding, modifications, and cleavage. Related references: PMID:34516797
• RPS28 localizes to the cytoplasm (cytosol, rough endoplasmic reticulum) and the nucleus (nucleolus), consistent with its roles in translation and ribosome assembly.
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomics identification and direct protein sequencing.
• RPS28 is also associated with Diamond-Blackfan anemia, a congenital disorder linked to defective ribosome biogenesis.
• It belongs to the ribosomal protein S28e family and is highly conserved across eukaryotes.
• The protein has a molecular weight of 7841 Da (UniProt) or approximately 8 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human RPS28 (amino acids 1-69), ensuring recognition of native protein. For Western blot, consider loading sufficient total protein due to its low molecular weight (~8 kDa); a 15% SDS-PAGE gel is recommended. Test antisera at dilutions ranging from 1:500 to 1:2000 for WB.
• For IF/ICC, fixation with methanol or paraformaldehyde may be considered, and permeabilization is needed for intracellular staining. Validate in the relevant cell line or tissue sample, as expression is ubiquitous.
• For ELISA, the antibody can be used as capture or detection reagent; pairing with another anti-RPS28 clone may be explored for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like anti-RPS28, and bulk ancillary reagents. As a one-stop sourcing bridge, we streamline procurement from concept to clinic, offering rare target antibody sourcing and technical consulting for ribosome biology and related disease research. Whether you need polyclonal antibodies for ELISA development or monospecific antibodies for immunohistochemistry, CamelBio ensures reliable supply chains to accelerate your diagnostic and research projects.