Anti-RPS27L Rabbit Polyclonal Antibody for WB, ELISA - Q71UM5

Core Product Specifications and Parameters

Biological Background: RPS27L Function and Localization

• RPS27L (Ribosomal protein eS27-like) is a component of the 40S small ribosomal subunit, also designated as 40S ribosomal protein S27-like.
• It is encoded by the RPS27L gene in humans (Gene ID: 51065).
• The protein contains a zinc-finger domain and is classified as a metal-binding protein, indicating a role in zinc-dependent ribosomal functions.
• According to UniProt, RPS27L is a member of the reference proteome and has been identified through large-scale proteomics studies.
• The calculated molecular weight of RPS27L is approximately 9.5 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, note the theoretical molecular weight of ~9 kDa; use high-percentage gels and optimize transfer conditions for small proteins.
• For ELISA, titrate the antibody to determine the optimal coating concentration for your specific assay format.
• As a polyclonal antibody, lot-to-lot variability is possible; consider validating each new batch in your sample system.
• Cross-reactivity with mouse and rat homologs is expected based on supplier data, but confirm specificity in the target tissue or cell line.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers focusing on ribosomal proteins like RPS27L, we can facilitate access to rare-target raw materials and provide tailored technical consultation to support your diagnostic or research workflows.