Anti-RPS27A Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62979

Core Product Specifications and Parameters

Biological Background: RPS27A Function and Localization

• RPS27A encodes a ubiquitin-ribosomal protein fusion that is post-translationally processed to yield free ubiquitin and the ribosomal protein eS31 (also known as S27A), both of which play essential roles in cellular homeostasis.
• The ubiquitin moiety from this precursor participates in diverse ubiquitin-dependent processes, including protein degradation via Lys-48-linked polyubiquitin chains targeting substrates to the proteasome, as well as non-proteolytic functions such as DNA repair (Lys-6-linked), endocytosis and NF-kappa-B signaling (Lys-63-linked), and cell-cycle regulation (Lys-11- and Lys-29-linked).
• Ribosomal protein eS31 is an integral component of the 40S small ribosomal subunit and contributes to the assembly of the small subunit (SSU) processome in the nucleolus, where it aids in pre-rRNA folding, modification, and cleavage during ribosome biogenesis. Related references: PMID:23636399 PMID:9582194 PMID:34516797
• The protein localizes to both the cytoplasm and the nucleus, with enrichment in the nucleolus, consistent with its dual roles in ubiquitin signaling and ribosome production.
• Post-translational modifications of the fusion protein include acetylation, ADP-ribosylation, and phosphorylation, which may regulate its processing, stability, or interactions.
• RPS27A contains a zinc-finger domain and metal-binding motifs, suggesting structural roles in ribosomal assembly or ubiquitin-related functions.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length fusion protein (amino acids 1–156); the resulting polyclonal antibody may recognize epitopes within ubiquitin, eS31, or both. In Western blotting, consider that bands corresponding to the uncleaved fusion (~18 kDa), free ubiquitin (~8.5 kDa), and eS31 (~15 kDa) could appear depending on the sample's ubiquitin processing status and the epitopes recognized.
• For IHC-P and IF/ICC, the expected subcellular staining pattern includes cytoplasmic, nuclear, and nucleolar signals. Validate localization by co-staining with markers for these compartments.
• In ELISA applications, use recombinant full-length RPS27A or purified ubiquitin/eS31 as coating antigens to assess antibody specificity and titer.
• Cross-reactivity with mouse and rat is expected; however, always verify performance in your specific model system with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. For research on ubiquitin pathways and ribosomal biology, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Whether you need this RPS27A antibody for assay development or are seeking complementary reagents like recombinant ubiquitin, proteasome components, or ribosomal antigens, CamelBio is your trusted procurement partner.