Anti-RPS27A Polyclonal Antibody for WB, IHC-P, ELISA - P62979

Core Product Specifications and Parameters

Biological Background: RPS27A Function and Localization

• RPS27A encodes a fusion protein composed of N-terminal ubiquitin and C-terminal ribosomal protein eS31 (S27a), which is post-translationally cleaved to yield free ubiquitin and the ribosomal subunit.
• The ubiquitin moiety can be conjugated to target proteins, forming polyubiquitin chains that signal proteasomal degradation (Lys-48-linked) or mediate non-degradative processes such as DNA repair and NF-kB signaling (Lys-63-linked).
• As a ribosomal protein, S27a is a structural component of the 40S small ribosomal subunit and participates in pre-rRNA processing and ribosome assembly. Related references: PMID:23636399, PMID:34516797
• The protein localizes to the cytoplasm, nucleus, and nucleolus, aligning with its dual functions in translation and ubiquitin signaling.
• Post-translational modifications of RPS27A include acetylation, phosphorylation, and ADP-ribosylation.
• RPS27A belongs to the ubiquitin family and the eukaryotic ribosomal protein eS31 family.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human RPS27A; note that endogenous cleavage may generate multiple species potentially detectable by this polyclonal antibody.
• For Western blot, a band at ~18 kDa corresponding to the full-length fusion protein may be observed; validate in the relevant sample system and fractionation conditions.
• This antibody is tested for WB and IHC-P; for ELISA, consider using it as a capture or detection reagent after confirming reactivity with your target antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines access to high-quality IVD raw materials, connecting diagnostic manufacturers, labs, and research institutes with validated antibodies and ancillary reagents. This anti-RPS27A polyclonal antibody supports studies on the ubiquitin-proteasome system and ribosomal biogenesis, and we can further supply optimized monoclonal/polyclonal antibodies, bulk antigen-grade proteins, and custom sourcing for rare targets. Let us help bridge your research from concept to clinic.