Anti-RPS27 Rabbit Polyclonal Antibody for WB, ELISA - P42677

Core Product Specifications and Parameters

Biological Background: RPS27 Function and Localization

• Small ribosomal subunit protein eS27, also designated 40S ribosomal protein S27, Metallopan-stimulin 1 (MPS-1), and RPS27.
• Essential component of the small ribosomal subunit responsible for cellular protein synthesis. Related references: PMID:23636399, PMID:8706699
• Required for proper rRNA processing and maturation of 18S rRNAs. Related references: PMID:25424902
• Part of the small subunit (SSU) processome, where it contributes to pre-rRNA folding, modifications, and cleavage. Related references: PMID:34516797
• Subcellular localization includes cytoplasm, nucleus, and nucleolus.
• Expressed in a wide variety of actively proliferating cells and tumor tissues.
• Contains a zinc-finger domain and binds zinc; subject to phosphorylation.
• Associated with Diamond-Blackfan anemia (DBA17).

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human RPS27 (amino acids 1-84), covering the entire mature protein.
• For Western blotting, consider using high-percentage polyacrylamide gels and optimized transfer conditions due to the small protein size (~9 kDa).
• Validate antibody performance in human and rat samples, as cross-reactivity has been demonstrated.
• For ELISA, explore both direct and sandwich formats, and include appropriate controls for target abundance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers and researchers from early-stage concept to clinical application. For targets like RPS27—critical in ribosome biology and associated with Diamond-Blackfan anemia—CamelBio offers validated antibody pairs, optimized polyclonal/monoclonal antibodies, bulk ancillary reagents, and access to rare-target raw materials to streamline your procurement and assay development workflows.