Anti-RPS25 Polyclonal Antibody for WB, IF/ICC, ELISA - P62851

Core Product Specifications and Parameters

Biological Background: RPS25 Function and Localization

• RPS25 is a core component of the 40S small ribosomal subunit, essential for the assembly and function of the ribosome, the molecular machine that synthesizes proteins in all cells. Related references: PMID:23636399
• The eS25 protein is predominantly localized in the cytoplasm, where it participates in translation.
• RPS25 belongs to the S25e family of ribosomal proteins, conserved across eukaryotes.
• The protein undergoes post-translational modifications, including acetylation, as indicated by proteomics data.
• Additional keywords highlight its role as a ribonucleoprotein and reference proteome status, confirming its well-characterized nature in human biology.
• The predicted molecular weight of human RPS25 is approximately 13.7 kDa (UniProt), consistent with the observed 14 kDa band in experimental settings.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight of RPS25 is ~14 kDa; ensure appropriate gel percentage and transfer conditions for low molecular weight proteins.
• In immunofluorescence (IF/ICC), confirm specificity in relevant cell lines; RPS25 shows cytoplasmic staining.
• For ELISA, consider using the immunizing peptide as a positive control to validate antibody reactivity.
• Since the immunogen targets the C-terminal region, this antibody may recognize both full-length and truncated forms of RPS25.
• Validate cross-reactivity in mouse and rat samples; sequence conservation suggests detection in these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and labs with a one-stop sourcing platform for IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This anti-RPS25 polyclonal antibody is part of our extensive catalog supporting ribosomal protein research and translation-related studies. For custom sourcing of rare targets or technical consultation, CamelBio offers end-to-end support from concept to clinic.