Anti-RPS24 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - P62847

Core Product Specifications and Parameters

Biological Background: RPS24 Function and Localization

• RPS24 encodes eS24, a core component of the small ribosomal subunit essential for protein translation.
  Related references: PMID:23636399
• Required for processing of pre-rRNA and maturation of the 40S ribosomal subunit.
  Related references: PMID:18230666
• Functions in the SSU processome, mediating early ribosome biogenesis steps such as pre-rRNA folding, modification, and cleavage.
  Related references: PMID:34516797
• Localizes to the cytoplasm, nucleus, and nucleolus, reflecting its dual role in ribosome assembly and cytoplasmic translation.
• Highest expression in proliferating tissues (fetal brain, placenta, bone marrow), with low levels in mature tissues (adult brain, skeletal muscle, heart, kidney).
• Mutations in RPS24 are linked to Diamond-Blackfan anemia (DBA), a congenital ribosomopathy.
• Undergoes multiple post-translational modifications, including acetylation, phosphorylation, and isopeptide bond formation (ubiquitination).

Experimental Guidance and Technical Tips

• The antibody was raised against the N-terminal region (aa 1-130) of human RPS24; it may recognize full-length and certain alternative splice isoforms. Validate in your specific biological system.
• For Western blotting, a band near 15 kDa (calculated ~15.4 kDa) is expected. Use high-percentage SDS-PAGE gels to resolve low-molecular-weight proteins effectively.
• In IHC-P, antigen retrieval conditions can influence detection; test with known positive controls like liver or testis, where RPS24 is abundant.
• The antibody cross-reacts with mouse and rat; verify species compatibility if applying to other organisms.
• Consider titration for optimal signal-to-noise ratio in your assay, as performance may vary with sample type and protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive IVD raw-material solutions, from validated antibody pairs and bulk ancillary reagents to rare target antigen sourcing. The Anti-RPS24 Rabbit Polyclonal Antibody supports investigations into ribosome biology, protein translation, and Diamond-Blackfan anemia pathobiology. For your IVD development needs, explore our full portfolio of monoclonal antibodies, recombinant proteins, and custom polyclonal generation to streamline your pipeline from concept to clinic.