Anti-RPS23 Polyclonal Antibody for WB, ELISA - P62266

Core Product Specifications and Parameters

Biological Background: RPS23 Function and Localization

• RPS23 encodes the 40S ribosomal protein S23, also known as uS12, a component of the small ribosomal subunit essential for protein synthesis. Related references: PMID:23636399, PMID:25901680, PMID:25957688
• It is part of the small subunit (SSU) processome, which assembles in the nucleolus and is critical for pre-rRNA processing and ribosome biogenesis. Related references: PMID:34516797
• RPS23/uS12 is localized in the cytoplasm, rough endoplasmic reticulum, and nucleolus, reflecting its role in both ribosome assembly and active translation.
• The protein is subject to post‑translational modifications including acetylation, hydroxylation, and ubiquitination (isopeptide bond), as indicated by UniProt keywords.
• Mutations in RPS23 are associated with human diseases such as deafness and dwarfism (keywords from UniProt).
• RPS23 plays a critical role in maintaining translational accuracy by influencing codon–anticodon interactions. Related references: PMID:28257692

Experimental Guidance and Technical Tips

• The recombinant immunogen (amino acids 40–120 of human RPS23) corresponds to a conserved region of the protein; consider sequence alignment with target species to assess expected reactivity.
• For Western blot (WB), the predicted molecular weight of RPS23 is ~16 kDa; ensure appropriate gel percentage and transfer conditions for small proteins.
• In ELISA applications, optimize antibody dilution by checkerboard titration using the relevant sample matrix.
• As a polyclonal antibody, batch-to-batch variation may occur; validate in the specific sample system.
• Given the high evolutionary conservation of ribosomal proteins, cross-reactivity with other species is plausible but was only confirmed for human; validate experimentally if other species are tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, serving as a one-stop bridge from concept to clinic. This anti-RPS23 polyclonal antibody joins our expanding catalog of translation and ribosome biology reagents, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For projects requiring rare or custom ribosomal protein targets, CamelBio offers tailored raw-material sourcing to accelerate your assay development and registration.