Anti-RPS21 Rabbit Polyclonal Antibody for WB, ELISA - P63220

Core Product Specifications and Parameters

Biological Background: RPS21 Function and Localization

• RPS21 encodes the 40S ribosomal protein S21, also known as eS21, which is a component of the small ribosomal subunit.
• It plays a critical role in protein synthesis by participating in the ribosome's translational machinery (PubMed:23636399, PubMed:25901680, PubMed:25957688). Related references: PMID:23636399, PMID:25901680, PMID:25957688
• The ribosome is a large ribonucleoprotein complex that catalyzes protein synthesis, and RPS21 is integral to its 40S subunit.
• RPS21 localizes to the cytoplasm, including the cytosol and rough endoplasmic reticulum, consistent with its role in translation.
• Post-translational modifications of RPS21 include acetylation and ubiquitin-like conjugation (Ubl conjugation), as identified by proteomics.
• The protein has a molecular weight of approximately 9.1 kDa (calculated from UniProt).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein encompassing the full-length human RPS21 sequence (amino acids 1-81). This design may offer comprehensive epitope recognition by the polyclonal antibody.
• Recommended applications include Western blot (WB) and ELISA, as validated by the supplier. For WB, a band around 9 kDa is expected.
• Cross-reactivity with mouse RPS21 is reported; therefore, this antibody can be considered for studies in mouse tissues or cell lines, but validation in the relevant mouse system is advisable.
• Because the antibody is a polyclonal, it is important to confirm specificity in knockout or knockdown experiments to avoid off-target binding.
• For ELISA, optimizing coating antigen concentration and antibody dilution is recommended to achieve the best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. For ribosomal biology and protein synthesis studies, we supply validated antibody pairs, optimized polyclonal antibodies like this RPS21 pAb, and bulk ancillary reagents. Our sourcing network also handles rare target raw-material procurement to support your IVD development pipeline.