Anti-RPS20 Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P60866

Core Product Specifications and Parameters

Biological Background: RPS20 Function and Localization

• RPS20, also known as 40S ribosomal protein S20 or uS10, is a key component of the small (40S) ribosomal subunit. It participates in the ribosome's essential function of protein biosynthesis. Related references: PMID:23636399
• The RPS20 gene undergoes alternative splicing, which may result in multiple isoforms contributing to ribosome heterogeneity.
• RPS20 is subject to various post-translational modifications, including acetylation, phosphorylation, and ubiquitination, which may regulate ribosome function or protein stability.
• Localized primarily in the cytoplasm, RPS20 is part of the ribosomal complex that translates mRNA into protein.
• RPS20 is a member of the universal ribosomal protein uS10 family, conserved across eukaryotes and archaea.

Experimental Guidance and Technical Tips

• The immunogen sequence corresponds to the N-terminal region (aa 1-100), which is highly conserved between human, mouse, and rat, consistent with the observed cross-reactivity. For other species, verify sequence homology before use.
• For Western blot, the predicted band is around 13 kDa; consider using a high-percentage gel (e.g., 12-15%) for optimal resolution of low molecular weight proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), include both positive and negative tissue/cell controls due to ubiquitous expression of ribosomal proteins.
• For immunoprecipitation (IP), the native conformation of the epitope may be critical; validate in the relevant sample system.
• ELISA applications may require optimization of coating antigen and detection conditions; the peptide immunogen can serve as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering comprehensive solutions from concept to clinic. This anti-RPS20 monoclonal antibody supports studies on ribosome function and protein synthesis, and our supply capabilities include validated antibody pairs, optimized monoclonals, bulk reagents, and customized sourcing for rare targets. Partner with us to accelerate your diagnostic or research workflow.