Anti-RPS19BP1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q86WX3

Core Product Specifications and Parameters

Biological Background: RPS19BP1 Function and Localization

• Also known as AROS (Active Regulator of SIRT1) and S19BP, RPS19BP1 is a 40S ribosomal protein S19-binding protein that functions both in ribosome biogenesis and as a direct regulator of SIRT1 deacetylase activity.
• As a component of the small subunit (SSU) processome in the nucleolus, RPS19BP1 acts as a chaperone that specifically mediates the integration of RPS19 into the pre-40S ribosomal subunit during late steps of ribosome assembly. Related references: PMID:34516797
• RPS19BP1 directly binds and enhances SIRT1 deacetylation of p53/TP53, leading to inhibition of p53-mediated transcriptional activity and implicating the protein in cell survival and stress response pathways. Related references: PMID:17964266
• The protein localizes predominantly to the nucleus and nucleolus, consistent with its roles in ribosomal RNA processing and transcriptional regulation.
• RPS19BP1 is widely expressed at the protein level across different tissues.
• Post-translational modifications reported for RPS19BP1 include citrullination and phosphorylation (UniProt keywords), and its three-dimensional structure has been resolved, providing insights into its molecular interactions.

Experimental Guidance and Technical Tips

• Based on the immunogen design (aa 1-136), the antibody is expected to recognize the full-length RPS19BP1 protein. For western blotting, a band near 15 kDa should be observed; however, actual migration may vary depending on gel and buffer conditions.
• For immunofluorescence/ICC, anticipate nuclear and nucleolar staining. Validate the pattern in relevant positive cell lines (e.g., HeLa, HEK293) and consider co-staining with nucleolar markers to confirm localization.
• When performing ELISA, ensure proper coating of the target protein and validate cross-reactivity with mouse and rat orthologs if needed.
• Include appropriate positive and negative controls in all experiments to confirm antibody specificity, as polyclonal antibodies may exhibit lot-to-lot variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including this anti-RPS19BP1 polyclonal antibody for ribosomal biogenesis and SIRT1/p53 pathway investigations. We offer validated monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents in bulk, along with technical consultation to streamline your assay development and production workflows. Should you require specialized raw-material sourcing or customized solutions, our team is ready to bridge your project from concept to clinic.