Anti-RPS19 Polyclonal Antibody for WB, ELISA - P39019

Core Product Specifications and Parameters

Biological Background: RPS19 Function and Localization

• Encodes the 40S ribosomal protein S19 (eS19), a component of the small ribosomal subunit essential for protein synthesis. Related references: PMID:23636399
• Required for pre-rRNA processing and maturation of 40S ribosomal subunits, playing a critical role in ribosome biogenesis. Related references: PMID:16990592
• Part of the small subunit (SSU) processome, the first precursor of the small eukaryotic ribosomal subunit, where it contributes to RNA folding, modifications, and cleavage. Related references: PMID:34516797
• Localizes to the cytoplasm, nucleus, and nucleolus, reflecting its involvement in both ribosome assembly and mature ribosome function.
• Exhibits higher expression in colon carcinoma tissue compared to normal colon tissue, suggesting a potential role in cancer biology.
• Undergoes post-translational modifications including acetylation and methylation, which may regulate its function or interactions.
• Associated with Diamond-Blackfan anemia (DBA), a congenital bone marrow failure syndrome, emphasizing its clinical significance in hematopoiesis.
• Implicated in host-virus interactions, as indicated by keyword annotation.

Experimental Guidance and Technical Tips

• The immunogen was designed from the full-length human RPS19 protein (amino acids 1–145), which may provide strong reactivity in WB and ELISA applications; consider validating the antibody in your specific sample system.
• Due to the predicted molecular weight of ~16 kDa, ensure appropriate gel percentage (e.g., 12–15% SDS-PAGE) and transfer conditions (e.g., low molecular weight cutoff membrane, wet transfer at 100 V for 60 min) for Western blotting.
• This antibody is raised against human RPS19; cross-reactivity with other species has not been tested, so include species-matched controls if used in non-human samples.
• For ELISA, optimize coating antigen concentration and antibody dilution using a checkerboard titration to achieve the best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting your ribosomal protein, cancer biomarker, and Diamond-Blackfan anemia research. As a one-stop sourcing partner, we provide validated antibodies, optimized antibody pairs, bulk ancillary reagents, and rare-target raw-material procurement to streamline your diagnostic development from concept to clinic.