Anti-RPS19 Monoclonal Antibody for WB, IP, IF/ICC, IF-P, ELISA - P39019

Core Product Specifications and Parameters

Biological Background: RPS19 Function and Localization

• RPS19 (40S ribosomal protein S19) is a component of the small ribosomal subunit and is essential for protein synthesis in the cell.
  Related references:
  PMID:23636399
• It is required for pre-rRNA processing and maturation of 40S ribosomal subunits and participates in the assembly of the small subunit processome in the nucleolus.
  Related references:
  PMID:16990592 PMID:34516797
• The protein localizes to the cytoplasm, nucleus, and nucleolus, reflecting its dynamic role in ribosome biogenesis.
• Post-translational modifications such as acetylation and methylation have been identified, which may regulate ribosomal function.
• Higher expression of RPS19 is observed in colon carcinoma tissue compared to normal colon tissue, suggesting a potential role in cancer biology.
• Mutations in RPS19 are linked to Diamond-Blackfan anemia, a congenital erythroid aplasia characterized by bone marrow failure.
• RPS19 is also involved in host-virus interactions, highlighting its multifunctional significance.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide spanning amino acids 66–145 of human RPS19; consider verifying epitope binding in your target species if cross-reactivity is critical.
• For Western blotting, expect a band at approximately 16 kDa under reducing conditions; titrate primary antibody concentration (e.g., 1:500–1:2000) and use appropriate loading controls.
• In immunoprecipitation and immunofluorescence applications, validate the antibody in positive controls (e.g., human, mouse, or rat cell lines) and include isotype controls.
• For ELISA, adhere to standard direct or indirect protocols and optimize coating concentrations and detection reagents for your specific assay setup.
• This antibody has been reported for use in formalin-fixed paraffin-embedded (IF-P) samples; test antigen retrieval methods to maximize signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and research institutes with a comprehensive range of IVD raw materials and technical support. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for targets like RPS19. From early-stage development to clinical applications, we streamline procurement and provide rare-target sourcing services to accelerate your project timelines.