Anti-RPS18 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62269

Core Product Specifications and Parameters

Biological Background: RPS18 Function and Localization

• RPS18, also known as 40S ribosomal protein S18 or Ke-3, is a core component of the small (40S) ribosomal subunit.
• It participates in the fundamental process of protein synthesis as part of the ribosome, a large ribonucleoprotein complex responsible for translating mRNA into proteins.
• The protein localizes to the cytoplasm, consistent with its role in ribosomal function.
• RPS18 possesses RNA-binding and rRNA-binding capabilities, essential for ribosome assembly and stability.
• Post-translational modifications, including acetylation and ubiquitination (isopeptide bond formation, Ubl conjugation), may regulate RPS18 function or turnover.
• High sequence identity across human, mouse, and rat highlights its evolutionary conservation in eukaryotic ribosomes.

Experimental Guidance and Technical Tips

• The full-length recombinant immunogen suggests recognition of multiple epitopes; consider using this antibody for total RPS18 detection.
• For Western blotting, expect a band near 18 kDa; confirm band size in your specific sample system and validate with appropriate controls.
• Cross-reactivity with mouse and rat allows rodent model studies, but verify specificity and performance in the relevant species and tissue.
• In IHC-P and IF/ICC, optimize antigen retrieval and dilution due to cytoplasmic localization; include proper controls.
• For ELISA, pair with a suitable detection antibody; note that polyclonal antibodies may exhibit broader reactivity, requiring careful validation.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we support diagnostic manufacturers and research teams by providing one-stop access to high-quality IVD raw materials for ribosome and protein synthesis research. In addition to anti-RPS18 antibodies, we offer validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate your assay development. From rare target sourcing to bulk supply, CamelBio bridges the gap between concept and clinic.