Anti-RPS17 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P08708

Core Product Specifications and Parameters

Biological Background: RPS17 Function and Localization

• RPS17 is a component of the small ribosomal subunit, essential for protein synthesis. Related references: PMID:23636399
• It is part of the small subunit (SSU) processome, the precursor of the small eukaryotic ribosomal subunit, and participates in pre-rRNA processing, folding, and modification during ribosome biogenesis. Related references: PMID:34516797
• Localized in the cytoplasm, nucleus, and nucleolus.
• RPS17 belongs to the ribosomal protein S17e family and is also known as 40S ribosomal protein S17.
• Mutations in RPS17 are associated with Diamond-Blackfan anemia, a disorder characterized by erythroid hypoplasia.
• The protein undergoes post-translational modifications including phosphorylation and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human RPS17. For Western blotting, the expected protein weight is ~16 kDa. Consider using reducing conditions for SDS-PAGE.
• For immunofluorescence (IF/ICC), RPS17 localizes to both cytoplasm and nucleus; permeabilization is required for nuclear access. Validate antibody specificity in the relevant sample system.
• ELISA may be employed for detection, but appropriate blocking and washing steps are essential to minimize non-specific binding.
• Cross-reactivity with mouse and rat allows for studies in common model organisms; however, confirm expression levels in the tissues/cells of interest.
• As a polyclonal antibody, batch-to-batch variability may exist; it is advisable to include positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For ribosomal protein and translation research, we offer a comprehensive portfolio of validated antibodies, including anti-ribosomal protein antibodies, matched antibody pairs, and bulk ancillary reagents. Whether you need optimized monoclonal/polyclonal antibodies or rare target raw-material sourcing, CamelBio is your trusted partner.