Anti-RPS14 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62263

Core Product Specifications and Parameters

Biological Background: RPS14 Function and Localization

• RPS14 encodes the small ribosomal subunit protein uS11, also known as 40S ribosomal protein S14, a core component of the 40S subunit.
• As part of the ribosome, RPS14 is involved in protein synthesis, specifically in the translation of mRNA into polypeptides.
• RPS14 is essential for the assembly of the small subunit (SSU) processome, the earliest precursor of the small ribosomal subunit. During this nucleolar process, RPS14, along with other factors, facilitates pre-rRNA folding, modification, rearrangement, cleavage, and targeted degradation by the RNA exosome. Related references: PMID:34516797
• RPS14 localizes predominantly to the cytoplasm and nucleus, with enrichment in the nucleolus, consistent with its role in ribosome biogenesis.
• The protein is subject to post-translational modifications, including phosphorylation and ubiquitin conjugation (isopeptide bond formation), which may regulate its function or assembly.
• According to UniProt, the mature RPS14 protein has a calculated molecular weight of approximately 16.3 kDa, consistent with the observed 16 kDa band in Western blots.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 1–151 of human RPS14, encompassing nearly the full-length protein, which may favor recognition of the native conformation.
• For Western blot (WB), a specific band at approximately 16 kDa is expected in human, mouse, and rat lysates under standard denaturing and reducing conditions.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), RPS14 typically displays both cytoplasmic and nucleolar staining patterns; validate fixation and permeabilization steps in the relevant sample system.
• For ELISA, consider titrating the antibody as a capture or detection reagent and verify specificity with recombinant RPS14 protein.
• While suggested starting dilutions (e.g., 1:500–1:2000 for WB, 1:50–1:200 for IHC/IF) commonly provide a useful range, empirical optimization for each application and sample type is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined pathway to high-quality IVD raw materials. As a dedicated sourcing partner, we provide validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and support for rare target procurement. This anti-RPS14 antibody supports investigations into ribosomal biology, protein synthesis, and nucleolus-related diseases, reinforcing our commitment to accelerating your project from concept to clinic.