Anti-RPS13 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62277

Core Product Specifications and Parameters

Biological Background: RPS13 Function and Localization

• RPS13 encodes the 40S ribosomal protein S13 (also designated uS15), a core component of the small ribosomal subunit.
• It is essential for protein synthesis, forming part of the ribosomal machinery that decodes mRNA and catalyzes peptide bond formation.
• During ribosome biogenesis, RPS13 is incorporated into the small subunit (SSU) processome in the nucleolus, where it aids in pre-rRNA folding, modification, and processing, working alongside RNA chaperones and processing factors (PubMed:34516797). Related references: PMID:34516797
• RPS13 is localized to both the cytoplasm (mature ribosomes) and the nucleus/nucleolus (assembly sites), reflecting its dual role in translation and ribosome production.
• The protein is subject to post-translational modifications, including acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), which may regulate its activity or stability.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 79-151) corresponds to a region of RPS13 that may be conserved across species, as supported by reported cross-reactivity with mouse and rat; verify in your specific experimental context.
• For Western blot (WB), the expected band size is ~17 kDa; use appropriate gel percentage and transfer conditions, and include positive controls (e.g., HeLa, HEK293, or liver lysates).
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and blocking conditions, and confirm signal specificity with knockdown/knockout controls or secondary-only controls.
• For ELISA, the antibody may be used as a capture or detection reagent; titrate in preliminary experiments to determine optimal working concentration.
• As a polyclonal antibody, lot-specific performance may vary; consider re-validating each new lot under your standard conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, academic laboratories, and research institutes with streamlined access to high-quality IVD raw materials. For ribosome biology and protein synthesis studies, CamelBio offers a comprehensive portfolio of validated antibodies, including this RPS13 polyclonal antibody, along with optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare and specialized targets. Our integrated supply chain supports your workflow from concept to clinic.